Directions for Use

It  is  a  violation  of  Federal  Law  to  use  this  product  in  a  manner  inconsistent with its labeling. PPM  is  a  broad-spectrum  preservative  and  biocide.  PPM  is  an excellent  preservative  agent  that  can  be  used  in  research  and  commercial  laboratories  to  inhibit  the  growth  of,  or  kill  bacteria  and  fungi  in  plant  tissue  culture  growth  media.  It  targets  fundamental enzymes in the Krebs cycle and in the Electron Transport Chain.  Depending  on  the  dose level,  PPM  is  both  biocidal  (>2ml/L of  media) and  Biostatic (<2ml/L  of  media).    When  diluted with  plant  growth  media  (5-20  ml/L)  PPM/liter  growth  media  is  effective as a micro-biocide against non-human health pathogenic organisms.

1. Media containing PPM may be dispensed outside the laminar flow hood (LFH) exposed to the ambient air. The plates should be covered  soon  after  agar  solidification.    In  the  event  a  pump  dispenses  the  media,  we  recommend  passing  autoclaved  hot  water through the hoses before and after dispensing media.

2. Heat  sensitive  or  heat  stable  liquid  media  containing  PPM does  not need to  be filter sterilized  or autoclaved  provided  that it will be stored in sterile containers and that the stock solutions are not contaminated. In rich media containing 200 mg/liter or more of amino acids or proteins, it is recommended to filter the media with the PPM.

3. Working  in  the  LFH  the  utensils  (forceps  or  scalpels)  do  not need to be flamed.  They may be periodically dipped in 70% alcohol. The LFH does not need to be certified and the work can also be  done  outside  the  LFH  on  a  clean  surface  for  a  period  not  exceeding 2 - 3 hours.

4. PPM comes in an acidic liquid solution (pH 3.8) and should be stored at 4ºC.  The recommended dose is 0.5 - 2.0 ml of PPM per liter  of  medium.    Higher  doses  are  required  to  treat  endogenous contamination or to obtain Agro-bacteria free plant material.

5. PPM is less effective when exposed to high density of bacteria or  fungi  spores  found  regularly  on  a  seed's  coat.    For  in  vitro germination,  seeds  should  be  conventionally  surface  sterilized with  EPA  registered  bleach.    Therefore,  in  the  presence  of  PPM (in  the  germination  medium),  the  seeds  can  be  rinsed  under  tap water in a non-sterile strainer and left to dry preferably in the LFH. Protoplast isolation solution should be sterilized mechanically through  Millipore  filters  with  the  PPM.    If  the  utensil  ends  have touched  active  bacteria,  fungi  culture  or  otherwise  suspected  of being  contaminated,  they  should  be  sterilized  by  autoclave  or  by use of an electric heating element.

6. Endogenous Contamination:  Plant  tissue  culture  media  containing PPM  at  doses  of  5-20  ml/l  can  be  used  to  eliminate  endogenous contamination  in  seeds  and  plant-explants.    In  such cases,  the  seeds  or  the  explants  should  be  treated  with  an  EPA registered plant disinfectant.  After rinsing with DD water, explants or buds should be embedded or placed in autoclaved semisolid or liquid  medium  respectively. The  proper  media  such  as  callus proliferation  or  regeneration  can  be  used  with  only  ¼  strength  of the inorganic salts, supplemented with 5-20 ml/l PPM/media mixture.  After 2-5 days the explants can be transferred without rinsing into a similar media (full strength inorganic salts) supplemented with at least 0.5 ml/l PPM at 20-24 degrees centigrade.  Seeds Can  be  transferred  to  germination  medium  (full  strength  of  inorganic salts) supplemented with 0.5 ml/l PPM after 5-10 days.

 It is up to the researchers to determine the optimal combination of PPM doses and time exposure.  Different plant types and different explant sources are highly varied in their response to PPM.   


Storage and Disposal

Do not contaminate water, food, or feed by storage and disposal. Storage: Ideal storage temperature is 39ºF (4º C).  Do not store at temperatures in excess of 70ºF (21ºC). 

Pesticide Disposal: Wastes resulting from the use of this product may be disposed of on site or at an approved waste disposal

Container Disposal: Nonrefillable container. Do not reuse or refill this container. Triple rinse as follows: Fill container 1/4 full with water and recap. Shake for 10 seconds. Drain for 10 seconds after the flow begins to drip. Follow Pesticide Disposal instructions for rinse disposal. Repeat procedure two more times. Then offer for recycling if available or puncture and dispose of in a sanitary landfill, or by incineration.