Skip to product information
1 of 3

DKW Medium w/ Vitamins

DKW Medium w/ Vitamins

Regular price $19.99 USD
Regular price $19.99 USD Sale price $19.99 USD
Off Sold out
Taxes included. Shipping calculated at checkout.
Size

For large bulk orders, contact sales@plantcelltechnology.com.

DKW medium is also known as Driver and Kuniyuki Walnut medium. The media was originally developed for walnut micropropagation using nodal explant. Notably, DKW medium contains lower nitrogen and higher sulfate content, potentially influencing plant growth and development.

The Benefits of DKW Media

  • Easy to use
  • Supply nutrients required by in vitro plants for their proper growth and development
  • Lower nitrogen content and higher sulfate content
  • Multiple shoot induction
  • Callus-mediated shoot regeneration

How To Use DKW Media

Follow the steps below to prepare 1 liter of tissue culture media using DKW media:

  1. Take a beaker and add 800 ml of distilled water to it.
  2. To the water add 5.36 grams of PCT’s DKW Media, 30 grams of sugar, and PPM™ (generally 1-2 ml/L).
  3. Make up the volume of the media to 1000ml.
  4. Adjust the pH of the media between 5.4-5.8.
  5. Add gelling agent (agar 6-8 g/L or gellan gum 3-4 g/L) to the media and keep stirring.
  6. Autoclave the medium for 20 minutes at 15 psi and 121℃.

Your media is ready!

 

Product Information

Product NameDKW Medium Basal Medium with Vitamins
SynonymsDriver and Kuniyuki Walnut medium
AppearanceWhite to light tan with a homogenous mixture of free-flowing powder
Formula weight5.36 g/L
SolubilityWater (Appear as colorless to slightly yellow solution, clear, complete)
SuitabilityPass for Tissue Culture
pH (at 25°C)4.8 ± 0.2
Storage and Shelf LifeDehydrated plant tissue culture media is hygroscopic and should be protected from sunlight and moisture. Store the prepared medium at 2-8°C away from direct light. Medium should be used before the expiry date. The entire volume of each bottle should be used immediately after opening, or else the unused portion should be stored in desiccators and refrigerated at 2-8°C.

Shipping & Storage

Storage Temp Room temperature, unless the medium is prepared. If medium is prepared, store iat 2-8 °C and away from direct light.
Shelf Life Check the label on the bottle for the expiry date of the product
HS Code

3824.99

Applications

Multiple shoot induction

Callus-mediated shoot regeneration

Improved success rates for specific plant species (e.g., turmeric and walnut)

Used to tissue culture woody plants

View full details

Batch Calculator

How many liters of medium do you need?

Liters of Water: 0
Quantity (250ml Vessels): 0

Customer Reviews

Be the first to write a review
0%
(0)
0%
(0)
0%
(0)
0%
(0)
0%
(0)