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Plant Preservative Mixture (PPM™)

Plant Preservative Mixture (PPM™)

Regular price $34.99 USD
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Plant Preservative Mixture (PPM™), is a broad-spectrum biocide for plant tissue culture. PPM™ is the ultimate solution to the never-ending struggle against microbial airborne, waterborne, and endogenous contamination.

Coupled with proper observation of aseptic laboratory techniques, PPM™ can increase your chances of a successful and thriving culture experiment.

The Benefits of Plant Preservative Mixture

  • At optimum doses, PPM™, which stands for Plant Preservative Mixture™, is an extremely effective Preservative/Biocide, yet does not impair in vitro seed germination, callus proliferation and callus regeneration.
  • PPM™ prevents the germination of both bacteria and fungi spores. It is heat stable and therefore can be autoclaved with the media.
  • PPM™ can be, and should be used as a standard ingredient in plant tissue culture media, and is also substantially less expensive than commonly used antibiotics.
  • While PPM™ was principally designed to inhibit airborne contamination, waterborne contamination, and contamination introduced from human contact, it can also -- in many cases -- be used to reduce endogenous contamination.
  • PPM™ does not affect the genetic profile of the plants.

How To Use Plant Preservative Mixture?

  1. General Dosage levels:

With the exception of endogenous contamination, the recommended dose range is 0.05%-0.2%. (For callus proliferation, organogenesis and embryogenesis, the recommended range is 0.05-0.075%.) To eliminate higher endogenous contamination densities, higher doses of PPM™ are needed.

  1. Endogenous Contamination:

(a) For explants: gently and routinely shake / stir 1 cm. long explants (or shorter) for 4-12 hours in 4-5% v/v PPM™ solution supplemented as above with 3x MS
strength basal salts without Tween 20.Without rinsing, insert into a medium supplemented with 0.05 - 0.1% PPM™ for herbaceous plants and 0.2% PPM™ for woody plants.  

Note: Paragraphs 6(b) through 10 below are intended for ornamental plants only.

(b)For tubers, bulbs and scales: shake / stir the entire tuber/ bulb / scale in bleach. Rinse with water (can be done under non-sterile conditions). Slice the tuber / bulb /scale to thin slices. Shake / stir for 12-24 hours in 4- 5% PPM™ solution supplemented with full strength basal salts without pH ing & Tween 20. Without rinsing, insert into a medium supplemented with 0.1 -0.2% PPM™.

Product NamePlant Preservative Mixture (PPM™)
SynonymsPPM™
FeatureAutoclavable and heat stable
UsePreservative for plant tissue culture media
pH5 - 7
CapacityAvailable in different volumes of 30mL, 100mL, 250mL, 500mL, and 1L.
ShapeCylindrical container
MaterialLiquid
ColorClear to light amber
OdorMild
SolubilityInfinite in water
SuitabilityFor Tissue Culture

Storage Temp 39° F (4° C).
Shelf Life

Check the product label for expiration date

HS Code 3808.94

  • Controller of microbial airborne contamination.
  • Manager of microbial waterborne contamination.
  • Inhibitor of endogenous contamination.
  • Enhancer of the success of culture experiments.
  • Maintainer of the viability of in vitro seed germination.
  • Promoter of callus proliferation.
  • Supporter of callus regeneration.
  • Ensurer of the stability of tissue culture media.
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PPM™ Usage Table

Contributor Plant  Name Contaminant ID PPM concentration (mL/L) Tissue Type/Application Result / Comments
Contributor: Dr. Ashish Dave Plant Name: Malus rootstock Contaminant ID: Fungi and Bacterial PPM Concentration (ML/L): 1ml/L Tissue Type/Application: added to media Result/Comments: PPM @1ml/L added to Malus rootstocks shoot multiplication media. It was very effective in controlling bacteria, without causing any phytotoxicity and growth aberrations.
Contributor: Richard P Plant Name: Hemp Contaminant ID: Fungi and Bacterial PPM Concentration (ML/L): 3% PPM Tissue Type/Application: Explants Result/Comments: We wash explants 3% PPM in sterile water for surface sterilization and 3% PPM in MS Media for shoot initiation
Contributor: Yaroslov Fuglevich Plant Name: Paulownia Contaminant ID: Bacteria and fungus PPM Concentration (ML/L): 1ml/L Tissue Type/Application: Use PPM in the culture media, added before sterilization Result/Comments: very effective, does not cause phytotoxicity
Contributor: Arthur P Plant Name: Philodendron Pink Princess Contaminant ID: Fungi and Bacterial PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: Explants Result/Comments: 40mL/L & 0.5mL/L philodendron pink princess Explants were washed in 4% PPM and were inoculated on media with 0.05% PPM no infections .
Contributor: Matt Miller Plant Name: Venus Flytrap Contaminant ID: Fungi and Bacterial PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: Explants and Seeds Result/Comments: Put PPM in media pre-autoclave. Dramatically reduces contamination on both seeds and explants.
Contributor: Patrick **** Plant Name: Ceropegia Genus Contaminant ID: Bacteria and Fungi PPM Concentration (ML/L): 2 mL/L Tissue Type/Application: Stem Explant Result/Comments: Added to media prior to autoclaving. Prevents contamination.
Contributor: Safa Labidi Plant Name: Potato Contaminant ID: Bacteria PPM Concentration (ML/L):  1ml/L Tissue Type/Application:  Shoot/Callus Result/Comments: used 1ml/l in our culture media to avoid contaminants
Contributor: Md Faruk Hossain Plant Name: Aloe Vera Contaminant ID: Rhizome PPM Concentration (ML/L): 2 mL/L Tissue Type/Application:   Result/Comments: Prior to autoclave
Contributor: Natalia Martinez-Ochoa Plant Name: Tobacco Contaminant ID: Fungal and bacterial PPM Concentration (ML/L): 2 mL/L Tissue Type/Application: Leaf midvein and callus tissue culture. PPM is added to tissue culture media when it is being prepared, before or after autoclaving Result/Comments: Very Effective in preventing contamination
Contributor: Hasnorita Abdul Rahman Plant Name: Pineapples Contaminant ID: Fungal PPM Concentration (ML/L): 2 mL/L Tissue Type/Application: Sucker Result/Comments: Put onto medium, very effective to reduce contamination rate
Contributor: Dr. Kamlesh Patel Plant Name: Lonicera Species Contaminant ID: Bacteria PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: Actively Growing Shoot Tips Result/Comments: Very Effective in preventing and eliminating bacteria in tissue culture
Contributor: Yengl Gukh Plant Name: Bean Contaminant ID: Fungus PPM Concentration (ML/L):  100ml purchased Tissue Type/Application: leaf Result/Comments: Incredible fungal removal effects were seen in callus cultures
Contributor: Jennie Plant Name: Coconut Contaminant ID: bacterial PPM Concentration (ML/L): 0.1 ml/L Tissue Type/Application: embryo Result/Comments:  0.1ml/L PPM were incorporated into tissue culture media
Contributor: Mareid Glediee Plant Name: Yuzus Contaminant ID: Bacteria PPM Concentration (ML/L): 2 ml/L Tissue Type/Application: Shoot Result/Comments:  It was used for disinfection when preparing sections in tissue culture
Contributor: MARIA LOURDES VICENTE Plant Name: Pepper Contaminant ID: bacteria PPM Concentration (ML/L):  50ml purchased Tissue Type/Application: anthers  Result/Comments:  surface sterilization, in media
Contributor: Petolescu Ceraselaz Plant Name: Rubus fruticosus Contaminant ID: bacteria and fungus PPM Concentration (ML/L): 2 mg/L Tissue Type/Application: I use PPM in the culture media, added before sterilization; Result/Comments:  very effective
Contributor: Eliseo Tesón Plant Name: Drosera,Nepenthes,Dionaea Contaminant ID: bacteria and fungal PPM Concentration (ML/L): 2 mL/L Tissue Type/Application: Leaf pullings and Seed sowing Result/Comments:  I do use PPM when other disinfection procedures do not work, for example leaf pullings in drosera. Also I do use it when I exchange flasks with other people that already used PPM as a preservative i do add PPM if i have to replace the seedlings. Also a rinse of PPM after sterilizing Nepenthes seed with NaDCC has proben to be very effective to kill the remaining bacterial contamination.
Contributor: Innan Godínez García Plant Name: Vanila planifolia (Vainilla) Orquídea Contaminant ID: bacteria, yeast, and fungi PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: 319/5000 The explants were washed with a Tween 20 1ml / L solution for 20 min outside the laminar flow hood. Inside the hood they were subjected to a 10% chlorine solution for 20 min and 2 drops of Tween 20 in 100ml of water. Then they were left in PPM for 10min. and were planted in MS 1/2 medium with 0.5 ml / L of PPM apical meristem and node Result/Comments: very effective 
Contributor: Carlos Urrutia Plant Name: Orchid Contaminant ID:   PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: apply it after sterilization Result/Comments:  
Contributor: Jesus Lucina Romero Plant Name: Blueberry Contaminant ID: bacterial PPM Concentration (ML/L): 0.5 mL/L Tissue Type/Application: Shoot Result/Comments: Add 0.5ml/L before sterile de medium
Contributor: Daniel Zavala Ortiz Plant Name: Tetrastigma Contaminant ID:   PPM Concentration (ML/L): 2 mL/L Tissue Type/Application: suspension culture Result/Comments:  Preventing contamination during bioreactor cultures
Contributor: Jeanmarie Molina Plant Name: Taxus Globosa S Contaminant ID:   PPM Concentration (ML/L): 0.5 mL/L Tissue Type/Application: stem, root Result/Comments: Preventing contamination during bioreactor cultures;
Contributor: Remi Bonnart Plant Name: Vitis (Grape) Contaminant ID: endophytic bacteria PPM Concentration (ML/L): 15 mL/L Tissue Type/Application: Nodal Segments; Result/Comments: Helpful to control endophytic bacteria in Vitis shoots taken from potted plants and introduced to tissue culture for several weeks with minimal/no toxic effects
Contributor: Hail Z. Rihan Plant Name: Cauliflower Contaminant ID:   PPM Concentration (ML/L): 0.5 mL/L Tissue Type/Application: Cauliflower microshoots Result/Comments: The use of 0.5 mL L−1 of PPM with culture medium was found to be effective in controlling contamination and keeping the growth capacity of microshoots.
Contributor: Matilde E. Uribe Plant Name: Traubia modesta Contaminant ID: Bacterial PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: Bulbs of T. modesta(Roots, the upper-third of the stem, and dry outer scales were removed) Result/Comments: Bacterial contamination could be efficiently controlled with streptomycin and PPM
Contributor: Dawood Ahmad Plant Name: Curcuma, Kaempferia and Zingiber Contaminant ID: Endogenous Contamination PPM Concentration (ML/L): 2 mL/L Tissue Type/Application: Buds Result/Comments: Preservative mixture (PPM) treatment which helped to minimize the endogenous contamination.
Contributor: Junji Miyazaki Plant Name: Petunia hybrida Contaminant ID:   PPM Concentration (ML/L): 5 mL/L Tissue Type/Application: Upper young axillary buds Result/Comments: 1. HPLC revealed that axillary buds from upper young nodes absorbed significantly more PPM compared with those from the lower nodes. 2. Indexation indicated successful eradication of bacterial contaminants from upper young axillary buds after vacuum-infiltration with 5 ml l−1 PPM
Contributor: Sergei Krasnyanski Plant Name: Cotton Contaminant ID:   PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: Cotton seeds Result/Comments: Plant preservative mixture rinses were also included in the sterilization procedures to improve the efficiency of tested protocols.
Contributor: Pedro Sansberro Plant Name: Ilex paraguariensis Contaminant ID:   PPM Concentration (ML/L): 3.5 mL/L Tissue Type/Application: Nodal segment explants of Ilex paraguariensis Result/Comments:  
Contributor: Timothy A. Rinehart Plant Name: Hydrangea macrophylla (Thunb.) Ser. and Hydrangea paniculata Contaminant ID:   PPM Concentration (ML/L): 2 mL/L Tissue Type/Application: Hydrangea seed Result/Comments: 0.5× Gamborgs solid media in conjunction with Plant Preservative Mixture (PPM), and by sterilizing seed with trichloro-s-triazinetrione (Trichlor). Assays of physiology were conducted by sterilizing seed.
Contributor: Junji Miyazaki Plant Name: Black kangaroo paw Contaminant ID:   PPM Concentration (ML/L): 5 mL/L (under vacuum) Tissue Type/Application: Infected axillary buds and basal-stem calli Result/Comments: Indexation of plantlets raised from PPM-treated tissues indicated successful eradication of the endophyte from basal-stem calli, and from shoots regenerated from them.
Contributor: Freddy Mora Plant Name: T. modesta Contaminant ID: Bacterial, Fungal PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: The bulbs of T. modesta Result/Comments: The contamination was only by fungi because the bacterial contamination could be efficiently controlled with streptomycin and PPM as biocides.
Contributor: Gustavo Zapata Plant Name: Carnivorous Plants (Dionaea) Contaminant ID: Fungus PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: Leaf Result/Comments: prepare media with MS
Contributor: Mike **** Plant Name: Ficus carica Contaminant ID:   PPM Concentration (ML/L): 2 mL/L Tissue Type/Application: Shoots Result/Comments: Media supplement and explant disinfestation
Contributor: Gemplant b.v.b.a Plant Name: Cortaderia Contaminant ID:   PPM Concentration (ML/L): 1-2 ml/L Tissue Type/Application: add to medium before autoclavation Result/Comments:  
Contributor: Omobolanle Ade-Ademilua Plant Name: Peperomia pelucida Contaminant ID: Endophytic bacteria, Fungi PPM Concentration (ML/L): 40mL/L and 0.75mL/L Tissue Type/Application: Leaf Result/Comments: 40mL/L was used in disinfecting explants and 0.75mL/L added to media (solid)
Contributor: Jorn Hansson Plant Name: Gerbera, orchids, perennials Contaminant ID:   PPM Concentration (ML/L): 1mL/L Tissue Type/Application: Shoots Result/Comments: mixed in media right after sterilization
Contributor: Issaac Reyes Plant Name: Cephalotus follicularis Contaminant ID: Fungus, Bacteria PPM Concentration (ML/L): 80mL/L Tissue Type/Application: Seeds Result/Comments: 80ml/l, Soak seeds in 0.8ml ppm/10ml water+1/3MS media, FREE PGR'S, Sucrose, No pH Adjust for 3-4 hrs. Desinfection for cephalotus follicualris seeds
Contributor: Margaret Clark Plant Name: Native hawaiian seeds Contaminant ID:   PPM Concentration (ML/L): 1mL/L Tissue Type/Application: Seeds Result/Comments: To blotter paper in petri dishes
Contributor: Dr. Kamlesh R. Patel Plant Name: Lonicera Contaminant ID: Bacterial PPM Concentration (ML/L): 1mL/L Tissue Type/Application: Shoot Result/Comments: Added to the culture medium
Contributor: Kun Wei Plant Name: Norway Spruce Contaminant ID: Bacterial PPM Concentration (ML/L): 0.5 mL/L Tissue Type/Application: Somatic Embryos Result/Comments: Prevent the possible infections from handling.
Contributor: Xinlu Chen Plant Name: Hornwort Contaminant ID: Fungus PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: leaves Result/Comments: controlling fungi
Contributor: Katrina Dlugosch Plant Name: Centaurea spp. Contaminant ID:   PPM Concentration (ML/L): 1 mL/L (1% by volume) Tissue Type/Application: Seeds Result/Comments: Germination of seeds
Contributor: Oleg Bosyy Plant Name: Vaccinium Contaminant ID:   PPM Concentration (ML/L): 1.5 mL/L Tissue Type/Application: Shoots Result/Comments: addition of PPM to the medium
Contributor: Issaac ****** Plant Name: Dionaea Muscipula Contaminant ID: Fungus, Bacteria PPM Concentration (ML/L): 2 mL/L Tissue Type/Application: Leaf Result/Comments: Add 2ml/l in MS media and sterilize.
Contributor: Dr. Raquel Folgado Plant Name: Aloe Contaminant ID:   PPM Concentration (ML/L): 0.05 - 0.1 mL/L Tissue Type/Application: Shoots Result/Comments:  
Contributor: Dr. Raquel Folgado Plant Name: Magnolia Contaminant ID:   PPM Concentration (ML/L): 0.05 - 0.1 mL/L Tissue Type/Application: Shoots Result/Comments:  
Contributor: Dr. Raquel Folgado Plant Name: Avocado Contaminant ID:   PPM Concentration (ML/L): 0.05 - 0.1 mL/L Tissue Type/Application: Shoots Result/Comments:  
Contributor: Dr. Chunsheng Lu Plant Name: Berries Contaminant ID:   PPM Concentration (ML/L): 0.5 - 5ml/L Tissue Type/Application: Shoot Tip Result/Comments:  
Contributor: Manmeet Singh Plant Name: Potato Contaminant ID:   PPM Concentration (ML/L): 1.2ml/L Tissue Type/Application: Stems Result/Comments:  
Contributor: Martha Orozco Plant Name: Asperagus Contaminant ID:   PPM Concentration (ML/L): 10 mL/L Tissue Type/Application:   Result/Comments:  
Contributor: Martha Orozco Plant Name: Tomato Contaminant ID:   PPM Concentration (ML/L): 10 mL/L Tissue Type/Application:   Result/Comments:  
Contributor: Martha Orozco Plant Name: Rice Contaminant ID:   PPM Concentration (ML/L): 10 mL/L Tissue Type/Application:   Result/Comments:  
Contributor: Osagie Idehen Plant Name: Fern Contaminant ID:   PPM Concentration (ML/L): 1 mL/L Tissue Type/Application:   Result/Comments:  
Contributor: Osagie Idehen Plant Name: Sweet potato Contaminant ID:   PPM Concentration (ML/L): 1 mL/L Tissue Type/Application:   Result/Comments:  
Contributor: Jayakumar Pon Samuel Plant Name: Maize Contaminant ID:   PPM Concentration (ML/L): 1mL/L Tissue Type/Application:   Result/Comments:  
Contributor: Jayakumar Pon Samuel Plant Name: Soybean Contaminant ID:   PPM Concentration (ML/L): 1 mL/L Tissue Type/Application:   Result/Comments:  
Contributor: Jayakumar Pon Samuel Plant Name: Canola Contaminant ID:   PPM Concentration (ML/L): 1mL/L Tissue Type/Application:   Result/Comments:  
Contributor: Mr. Micah Stevens Plant Name: Juglans Contaminant ID:   PPM Concentration (ML/L): 2 mL/L Tissue Type/Application: Shoots Result/Comments:  
Contributor: Dr. Jiping Zhao Plant Name: Petunia Contaminant ID:   PPM Concentration (ML/L): 2 mL/L Tissue Type/Application: Leaf Result/Comments:  
Contributor: Dr. Qingzhen Jiang Plant Name: Alfalfa Contaminant ID:   PPM Concentration (ML/L): 3 – 5 mL/L Tissue Type/Application: Leaf Result/Comments:  
Contributor: Dr. Qingzhen Jiang Plant Name: Medicago Contaminant ID:   PPM Concentration (ML/L): 3 – 5 mL/L Tissue Type/Application: Leaf Result/Comments:  
Contributor: Dr. Qingzhen Jiang Plant Name: Switchgrain Contaminant ID:   PPM Concentration (ML/L): 3 – 5 mL/L Tissue Type/Application: Leaf Result/Comments:  
Contributor: Chetty Venkaterwen Plant Name: Rice Contaminant ID:   PPM Concentration (ML/L): 3.5 mL/L Tissue Type/Application: Seed/Embryo Result/Comments:  
Contributor: Chetty Venkaterwen Plant Name: Ciban Contaminant ID:   PPM Concentration (ML/L): 2 mL/L Tissue Type/Application: Leaf/Shoot Result/Comments:  
Contributor: Chetty Venkaterwen Plant Name: Tomato Contaminant ID:   PPM Concentration (ML/L): 2.5 mL/L Tissue Type/Application: Seedlings Result/Comments:  
Contributor: Kaitlin J. Palla Plant Name: Clusia Contaminant ID:   PPM Concentration (ML/L): 5 mL/L Tissue Type/Application:   Result/Comments:  
Contributor: Kaitlin J. Palla Plant Name: Kalanchoe Contaminant ID:   PPM Concentration (ML/L): 5 mL/L Tissue Type/Application:   Result/Comments:  
Contributor: Kaitlin J. Palla Plant Name: Poplar Contaminant ID:   PPM Concentration (ML/L): 5mL/L Tissue Type/Application:   Result/Comments:  
Contributor: Jim Bo Plant Name: Orchids Contaminant ID:   PPM Concentration (ML/L): 0.3 mL/L Tissue Type/Application: Nodes Result/Comments: Growing orchids
Contributor: RUANGWIT PORRUAN Plant Name: Nepenthes Contaminant ID:   PPM Concentration (ML/L): 1-2 mL/L Tissue Type/Application:  Shoot Result/Comments: Add 1-2 mL/L PPM to media before sterilization.
Contributor: Annie Lachapelle Plant Name: Rosa hybrida Contaminant ID:   PPM Concentration (ML/L): 10 mL/L Tissue Type/Application:  Shoot Result/Comments: Mix with culture
Contributor: Quoirin M. Plant Name: Bambusa Contaminant ID: Fungi and bacteria PPM Concentration (ML/L): 3 ml/L Tissue Type/Application: shoot segment Result/Comments: Desinfestation for introduction in vitro
Contributor: armin mustafic Plant Name: orchid Contaminant ID:   PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: leaf Result/Comments: Great, five star product!
Contributor: Dr. Kamlesh R. Patel Plant Name: Hosta Contaminant ID:   PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: bulb Result/Comments: Liquid Culture
Contributor: Naoaki Okamoto Plant Name: Nepenthes Contaminant ID:   PPM Concentration (ML/L): 5ml/L Tissue Type/Application: seed Result/Comments: In vitro culture of Nepenthes
Contributor: Marc Martinez Plant Name: Sarracenia Contaminant ID: Bacterial PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: seed Result/Comments: Bacterial and fungal prevention for in vitro multiplication.
Contributor: William Conner Plant Name: Citrus Contaminant ID:   PPM Concentration (ML/L): 4 mL/L Tissue Type/Application: Shoot / Tip Result/Comments: eliminates fungus, does not seem to have effect on plant
Contributor: Michelle Steenberghen Plant Name: Cortaderia Contaminant ID:   PPM Concentration (ML/L): 1.5ml/l Tissue Type/Application: shoots Result/Comments: add before autoclavation
Contributor: Choo ******** Plant Name: OIL Contaminant ID:   PPM Concentration (ML/L): 40mL/L & 0.5mL/L Tissue Type/Application: SEED Result/Comments: Explants were washed in 4% PPM and were inoculated on media with 0.05% PPM
Contributor: Erin ***** Plant Name: Canola Contaminant ID: fungal PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: callus, shoot Result/Comments: to be added to tissue culture media
Contributor: Dr. Kamlesh R. patel Plant Name: Elm Contaminant ID: Bacterial PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: shhot tips Result/Comments: In agar solidified medium.
Contributor: Hoang Tuan Vu Plant Name: Wasabi Contaminant ID:   PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: Shoot Result/Comments: Plant preservertive mixture
Contributor: Deborah McCown Plant Name: Amelanchier Contaminant ID: Bacteria and Fungi PPM Concentration (ML/L): 0.5 ml/L Tissue Type/Application: Shoot Culture Result/Comments: Tissue Culture media
Contributor: Maria Consue Medina Plant Name: blueberry Contaminant ID: Bacterial PPM Concentration (ML/L): 2 mL/l Tissue Type/Application: shoots Result/Comments: before autoclave in the culture media
Contributor: Carolina Rugeles Plant Name: Ornamentals Contaminant ID: Bacteria, Fungus PPM Concentration (ML/L): 2 mL/l Tissue Type/Application: Tissue Culture Result/Comments: Prepare in the Media solution
Contributor: Natalia Campistany Plant Name: cucumber Contaminant ID: Endogenus bacteria PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: plant Result/Comments: mixed in the solid media
Contributor: Obaid ur Rahman Plant Name: banana Contaminant ID: Fungal, bacterial PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: shoot tip cultures Result/Comments: PPM added at a concentration of 1 ml/L in liquid culture media and sterilized for 5 mins and 121 degrees centigrade
Contributor: Meenakshi Santra Plant Name: Wheat Contaminant ID:   PPM Concentration (ML/L): 0.5ml/l Tissue Type/Application: wheat embryo culture Result/Comments: we use in regeneration media and sometimes to kill the fungi in regenerated plants
Contributor: Desiree Ho Plant Name: Cantharanthus Contaminant ID:   PPM Concentration (ML/L): 0.01 mL/L Tissue Type/Application: Nodes and shoot meritsem Result/Comments: Preventing microbial contamination in tissue culture media.
Contributor: Joanna ***** Plant Name: maize Contaminant ID: Aspergillus PPM Concentration (ML/L): 2 mg/l Tissue Type/Application: embryo Result/Comments: Soke overnight
Contributor: Chan Woei Yee Plant Name: Anubias Contaminant ID: Bacterial PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: Corm Result/Comments: Plant Maintenance
Contributor: Bruno La Rosa Plant Name: Nepenthes Contaminant ID: Fungi spores, bacteria PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: Seed Result/Comments: 1 mL applied to 1 L of MS Basal medium mixture with sucrose and BAP.
Contributor: Joshua Spece Plant Name: Hosta Contaminant ID:   PPM Concentration (ML/L): 1-2 mL/L Tissue Type/Application: meristem bud Result/Comments: PPM used in the media. Greatly improves contamination rate.
Contributor: Julie ***** Plant Name: raspberry Contaminant ID:   PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: shoot Result/Comments: use on contaminated cultures that we must continue to multiply
Contributor: Maria Consue Medina Plant Name: Blueberry Contaminant ID: Bacterial PPM Concentration (ML/L): 2.5 mL/L Tissue Type/Application: Shoots Result/Comments: Before autoclave
Contributor: Gerardus VanDerMeij Plant Name: Impatiens Contaminant ID: Bacterial PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: Shoots Result/Comments: Used in propagation to keep cultures clean
Contributor: Viji ****** Plant Name: Orchids Contaminant ID:   PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: Shoot Result/Comments: Media preparation and cleaning tissues
Contributor: Md Faruk Hossain Plant Name: Aloe Vera Contaminant ID: Rhizome PPM Concentration (ML/L): 2 mL/L Tissue Type/Application:   Result/Comments: Prior to autoclave
Contributor: Leong MY Plant Name: Mango Contaminant ID: Leaf PPM Concentration (ML/L): 0.2 mL/L Tissue Type/Application: Cloning Result/Comments:  
Contributor:   Plant Name: Paulownia Contaminant ID: Bacterial and Fungus PPM Concentration (ML/L): 1 mL/L Tissue Type/Application: In the culture media and prior to sterilization Result/Comments: Very effective, does not cause phytotoxicity

Customer Reviews

Based on 52 reviews
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W
Will Utech
PTC Essential

Most aptly named product in the industry. Use when contamination shows up months later and you are too lazy to re-isolate.

L
Lynne McCormick

This brings Success. There is nothing else on the Market that compares

F
Fernando Pérez
Fungi killer

Excellent in reducing contamination in potato massive propagation.
Appart from that, excellent customer service and delivery

E
Edward Stec
Must have

I have been in TC for over 20 years, PPM has dramatically improved my success and significantly reduced contamination in my cultures Highly recommend
Ed

P
Peggy Martinez

Excellent customer service and quick shipping as always.