June 07, 2024

In-Person Tissue Culture Master Class

Tissue Culture Master Class
In-Person Tissue Culture Master Class

Hosted by Plant Cell Technology

Contact Host

Date and Time

Fri Jun 07 2024, 9:00 AM - Sun Jun 09 2024, 5:00 PM EST

Location PCT Tissue Culture Lab

Washington DC, USA

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Greg Borstelmann

Meet Greg B: Plant Tissue Culture Specialist, Founder of Shoots n’ Roots Labs, and Master Class Leader. Greg is committed to making success in plant tissue culture accessible to all, regardless of background. Recognizing the industry's training gaps, he established Shoots n’ Roots Labs to provide comprehensive and accessible training. Greg's masterclasses empower individuals at all expertise levels to succeed in plant tissue culture. Join Greg to gain the knowledge and solutions needed to grow, innovate, and overcome challenges in your tissue culture journey alongside like-minded enthusiasts.

In-Person Tissue Culture Master Class

Event description

Boost Your Plant Cultivation Game!

Transform your plant cultivation practices in just three days with our comprehensive Tissue Culture Master Class. Whether you're starting from scratch or looking to refine your techniques, this class is your gateway to mastering the art of tissue culture.

What You'll Gain:

  • A deep dive into tissue culture media preparation, including essential protocols and the sought-after list of equipment and materials.
  • Expert instruction on the entire tissue culture process, from initial plant cutting and explant preparation to advanced techniques such as meristem dissection for pathogen remediation and the use of synthetic seeds for long-term storage.
  • Practical lessons on the importance of plant hormones, macro and micronutrients, vitamins, organic compounds, and the right pH levels for successful micropropagation.
  • Hands-on experience with the latest in gender screening technology and strategies for effective micropropagation.
  • This Master Class, led by the Plant Cell Technology team and expert PCT Instructor Greg Borstelmann, is designed for growers, nursery owners, plant enthusiasts, and anyone keen on breaking into or advancing in the plant tissue culture industry.

Included with Your Enrollment:

  • Protocols and an equipment list designed to jumpstart your plant tissue culture operations.
  • Insights into media preparation, meristem dissection, and more, ensuring a well-rounded education.
  • A "Master Class Student Kit" featuring essentials like PPM™, agar, test tubes, and petri dishes, even a mini portable PCT flow hood, helping you put theory into practice immediately after the class. And if you are planning to fly, we can even ship it to you!

Don't miss this opportunity to elevate your plant cultivation with cutting-edge tissue culture techniques. Sign up now and start tissue culturing like a pro!

Keep on scrolling to find the itinerary and keep learning about this master class:

Tissue Culture Media Preparation

The foundation of successful plant tissue culture lies in the precise preparation of the growth media. This master class is designed to equip you with detailed guidelines, including:

  • Detailed Composition Guide: Master the art of media preparation by learning the exact requirements for macro and micronutrients, vitamins, and organic compounds, and their ideal concentrations for plant tissue culture.
  • pH Mastery: Understand the critical role of pH in tissue culture media and gain practical knowledge on accurately adjusting it for optimal growth conditions.
  • Pressure Cooker Essentials: Delve into the variety of pressure cookers, their applications in a laboratory setting, and how to select the best one for your tissue culture needs.
  • Laminar Flow Hood Introduction: Uncover the significance of laminar flow hoods in maintaining a sterile environment for tissue culture. This segment demystifies the equipment and its crucial role in your lab.
  • Plant Hormones Demystified: Navigate through the complex world of plant hormones with ease. This part explains their categories, the differences between natural and synthetic hormones, and their indispensable role in tissue culture.
  • Efficient Vessel Filling Techniques: Learn the optimal methods for filling culture vessels to ensure your plants receive the right amount of media, enhancing their growth and development.

By the end of this master class, participants will have built a robust foundation in the principles of tissue culture, equipped with the knowledge to create their own bespoke media. This tailored approach ensures the successful growth of plants in tissue culture, setting the stage for your cultivation success.

Leaf and Node Sterilization

In the crucial Plant Selection, Leaf, and Node Sterilization segment of our plant tissue culture master class, attendees are guided through the critical initial steps of choosing and preparing plant material for successful tissue culture. This comprehensive instruction includes:

  • Choosing the Right Plants: Gain expertise in selecting the optimal plants for tissue culture, focusing on disease-free specimens with desirable genetic traits. Learn strategies for gathering plant materials like leaves and nodes, ensuring minimal harm and maximal viability.
  • Sterilization Techniques Unveiled: Discover the spectrum of sterilization methods pivotal for tissue culture, including the use of bleach, ethanol, and other effective chemicals. This part emphasizes the significance of maintaining sterile conditions to prevent any form of contamination.
  • Practical Sterilization Application: Engage in practical exercises to master the cleaning and sterilization of plant leaves and nodes. This hands-on approach ensures you’re well-equipped to confidently and contamination-free bring your chosen plant materials into a tissue culture environment.

This section of the master class is designed to endow participants with both the theoretical understanding and practical skills necessary to select the ideal plant material and prepare it for tissue culture. By ensuring a sterile start, students can significantly increase their chances of successful cultivation, setting a strong foundation for their tissue culture endeavors.

Plant Micropropagation

In the Plant Micropropagation segment of our master class, students will dive deep into the advanced techniques of plant tissue culture. This part of the course is structured to cover essential areas such as:

  • Induction Techniques: Learn from our experts the nuances of initiating plant growth in tissue culture. This session covers the strategic application of plant growth hormones and the establishment of optimal environmental conditions that foster robust plant development.
  • Sterilization Mastery: Grasp the importance of sterilization to achieve success in plant micropropagation. Our teaching includes hands-on learning of the most effective surface sterilization techniques to rid your plant material of potential contaminants, ensuring a clean start for your cultures.
  • Practical Guide to Plant Cuttings: Understand the art and science of preparing plant cuttings. Our experts will guide you through the correct methods to cut and handle plant material, ensuring that explants remain clean, viable, and ready for the culture process.
  • Preparing Explants: Tackle the challenge of preparing both clean and potentially contaminated plant materials for culture. This comprehensive guide ensures you know how to secure clean, healthy plant tissues for introduction into tissue culture, minimizing the risk of contamination.
  • Essential Equipment Introduction: A bonus feature of this segment, this introduction to the necessary tissue culture equipment and tools sets you up for success. Whether starting a plant tissue culture venture or expanding your knowledge, being well-acquainted with the right tools of the trade is crucial.

This in-depth section of our master class is crafted to equip you with the knowledge, skills, and confidence to navigate the complexities of plant micropropagation. From induction to explant preparation, each topic is designed to bring you closer to mastering the art of plant tissue culture.

Re-sterilization and Subculture

In this pivotal segment of our plant tissue culture master class, participants will delve into the critical procedures of subculturing and resterilizing cultures, aimed at preserving the health and viability of plant cells for effective micropropagation. Key learning outcomes include:

  • The Role of Subculturing and Resterilization: Discover the significance of these processes in sustaining robust plant cell cultures, a foundation for successful micropropagation. Understanding why and when these steps are crucial can significantly impact the health of your cultures.
  • Mastering Aseptic Techniques: Gain insights into the meticulous steps of subculturing and resterilizing, with a strong emphasis on aseptic methods to ward off contamination. This instruction is vital for maintaining the purity and efficacy of your cultures.
  • Hands-On Practice: Under the expert supervision of our seasoned instructors, engage in practical sessions aimed at honing your skills in subculturing and resterilizing contaminated cultures. This hands-on approach ensures you're well-prepared to handle real-life challenges.
  • Troubleshooting Common Problems: Learn to identify and address frequent issues encountered during subculturing and resterilization, such as bacterial or fungal contamination. This knowledge is crucial for preemptively safeguarding your cultures against potential setbacks.
  • Best Practices for Culture Management: Elevate your understanding of the most effective strategies for managing plant cell cultures, ensuring their thriving growth and development. This comprehensive overview prepares you to apply these best practices in your own tissue culture endeavors.

Through this detailed examination of subculturing and resterilization techniques, our master class aims to equip you with the essential skills and knowledge to navigate the complexities of plant tissue culture successfully.

Meristem Pathogen Remediation

Plant tissue culture presents a unique set of challenges, particularly when it comes to the precise use of plant hormones. Achieving optimal growth and multiplication demands a nuanced understanding of which hormones to use, in what concentration, and the method of application. This masterclass offers an unparalleled opportunity to uncover these closely guarded secrets, shared by leading figures in the industry.

  • Deep Dive into Meristem Dissection: Meristem tissue, with its undifferentiated cells, holds the key to generating virus-free, genetically identical plant clones. This segment of our masterclass is dedicated to demystifying the process of meristem dissection:
  • Understanding Meristem Tissue: Learn about the crucial role meristem tissue plays in plant regeneration and how it can be harnessed to eliminate viruses from infected plants, ensuring the production of healthy clones.
  • Hands-On Meristem Dissection Techniques: Meristem dissection requires precision and expertise to avoid damaging the delicate tissues. Our industry experts will guide you through the process, providing hands-on experience and detailed instructions to master this technique.

This comprehensive exploration into the advanced techniques of plant tissue culture, including the strategic use of plant hormones and meristem dissection, is designed to elevate your cultivation practices to professional standards. Join us for this exclusive masterclass and transform your approach to plant tissue culture.

Seed Germination and Sterilization

This section of our master class is meticulously designed to empower students with the knowledge and skills essential for sustaining the health and viability of plant cell cultures for micropropagation. Here's what you can expect to learn:

  • The Critical Role of Subculturing and Resterilizing: Delve into the significance of these processes in maintaining robust plant cell cultures and ensuring the success of micropropagation efforts.
  • Step-by-Step Guidance on Subculturing and Resterilizing: Gain a comprehensive understanding of the procedures involved, including the adoption of aseptic techniques to ward off contamination. This part of the class emphasizes practical, actionable strategies to implement in your cultivation practices.
  • Hands-On Practice: Under the expert guidance of our seasoned instructors, engage in hands-on activities designed to refine your skills in subculturing and resterilizing, particularly focusing on cultures that have been compromised.
  • Troubleshooting Common Challenges: Learn how to swiftly identify and address issues like bacterial or fungal contamination, a critical skill set for any tissue culture enthusiast aiming for high success rates in plant cultivation.
  • Best Practices for Cultivation Success: Elevate your understanding of the overarching best practices in maintaining and managing plant cell cultures, setting a foundation for thriving micropropagation outcomes.

This comprehensive approach to subculturing and resterilizing in plant tissue culture is geared towards enhancing your technical proficiency and ensuring long-term cultivation success. Join us to unlock these vital skills, directly from the experts.

Synthetic Seeds

Embark on an exploration of cutting-edge preservation techniques in the plant tissue culture master class, with a spotlight on synthetic seeds for long-term storage. This session is dedicated to teaching you the art and science of using synthetic seeds, a revolutionary method for extending the shelf life of plant genetic material. Here's what you'll discover:

  • Understanding Synthetic Seeds: Dive deep into the concept of synthetic seeds, a novel approach designed to store plant tissues at lower temperatures efficiently. This method stands out for its ability to mimic natural seed dormancy and preservation qualities without the genetic variability.
  • Creating Synthetic Seeds: Gain hands-on experience in creating synthetic seeds using sodium alginate, a natural polymer that forms a gel-like substance when mixed with a calcium solution. This process encapsulates the plant material, effectively creating a "seed" that can withstand prolonged storage.
  • Encapsulation Techniques: Master the technique of encapsulating plant explants in the sodium alginate matrix. This crucial step ensures the plant material is adequately protected and ready for long-term storage, providing an optimal environment for later growth.
  • Benefits of Synthetic Seeds: Learn why synthetic seeds are a game-changer for plant cultivation. Their ease of transport, longevity, and capacity to preserve the genetic integrity of plant strains make them an invaluable tool for breeders and cultivators alike.
  • Exploring Additional Storage Methods: Beyond synthetic seeds, this segment of the master class will introduce you to other advanced storage techniques such as cryopreservation and slow growth storage. Understanding the advantages and limitations of each method will empower you to choose the best storage solution for your plant cultivation needs.

By the end of this comprehensive session, you'll be well-equipped with the knowledge and skills to implement synthetic seed technology in your plant cultivation practice. This innovative approach not only enhances the longevity and stability of your plant strains but also opens new avenues for research, breeding, and preservation in the ever-evolving world of plant tissue culture.

Elevate Your Cultivation Skills with Our Plant Tissue Culture Master Class

Dive into the world of plant tissue culture without the overheads of setting up a full-scale lab. Our Master Class offers comprehensive insights into plant tissue culture, opening doors to a wealth of information and the opportunity to network with industry leaders and fellow enthusiasts.

Upon completion, proudly display your Certificate of Achievement, a testament to your newly acquired expertise and dedication to the field.

Feedback from our previous session speaks volumes:

"We were blown away by the depth of knowledge and practical skills we gained. The hands-on experience was unparalleled, all at an incredible value. This has boosted our confidence in applying tissue culture techniques to enhance our operations." - Recent Attendee

Imagine being part of our dynamic "Tissue Culture Master Class: Plant," where learning translates into tangible skills for business growth and innovation.

Terms and Conditions:

Plant Cell Technology, Inc. prioritizes providing continuous, quality education. However, we reserve the right to modify or cancel classes if necessary. Affected participants will be notified and offered credits for future sessions.

Disclaimer: Participating in our Master Class signifies your understanding that Plant Cell Technology is not liable for personal injury or property damage.

Join us for an immersive learning experience that promises to be as enriching as it is educational. Secure your spot today and be part of the plant cultivation revolution!

Former Students Speak Out!

I am very grateful to have found PPM. In the past I was averaging a 70% contamination rate. Since using PPM I'm holding steady at a 5% contamination rate.

Peter Parker

Plant Cell Technology Customer

Extremely Pleased with Everything! Plant Cell Technology provided an amazing learning experience.

Student of Oct 2022 Master Class

Plant Cell Technology Customer

Extremely Pleased with Everything! Plant Cell Technology provided an amazing learning experience.

Student of Oct 2022 Master Class

Plant Cell Technology Customer

I loved it, it exceeded my expectations!

Student of January Master Class

Plant Cell Technology Customer

Additional Classes

Learn everything you need from the experts to start tissue culturing like a pro

In-Person Tissue Culture Master Class

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  • June 07 to 09, 2024
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Online LIVE Tissue Culture Master Class

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  • July 27 to 28, 2024
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