RPMI-1640 Medium - Modification 12

RPMI 1640 Medium - Modification 12 contains L-glutamine and 25 mM HEPES, but does not contain sodium bicarbonate--and is a versatile and essential medium widely utilized in animal cell culture, particularly for the growth and maintenance of various cell types. This enriched formulation is designed to provide an optimal environment that supports cellular metabolism, proliferation, and function. The addition of L-glutamine serves as a vital amino acid that promotes cell growth, while HEPES buffers maintain stable pH levels, ensuring a conducive environment for sensitive cells. This medium is ideal for applications such as immunology, cancer research, and bioproduction, enabling researchers to explore cellular responses, study disease mechanisms, and produce therapeutic proteins.

Key applications of RPMI 1640 Medium - Modification 12 include:

• Cultivation of lymphocytes for immune response studies
• Expansion of hybridoma cells for monoclonal antibody production
• Maintenance of cancer cell lines for drug screening and therapeutic studies
• Supporting primary cell cultures for various in vitro experiments

Supported animal cell types include:

• Human peripheral blood lymphocytes (PBLs)
• Mouse splenocytes
• Human epithelial carcinoma cells (e.g., HeLa)
• Chinese hamster ovary (CHO) cells
• Jurkat T cells

By this medium, researchers can enhance their experimental outcomes, driving innovations in biotechnology and medical research.

Disclaimer: Results may vary depending on specific cell lines, specifications, and experimental conditions. Consult with cell culture specialists and review comprehensive literature to tailor its use to your specific research needs.