RPMI 1640 Solution - Modification 2

RPMI 1640 Solution - Modification 2 does not contain L-glutamine and is a widely utilized culture medium in animal cell research, particularly effective for various cell types in immunology, oncology, and biotechnology. Its carefully balanced composition of nutrients, vitamins, and salts provides an optimal environment for cell growth and maintenance. The absence of L-glutamine allows researchers to tailor their experiments based on specific needs, particularly when using L-glutamine supplements or when studying cells that require different nitrogen sources.

Specific Applications:

• Immune Cell Culture: Ideal for the growth and activation of lymphocytes, facilitating the study of immune responses and vaccine development.
• Cancer Research: Supports the culture of various tumor cell lines for drug testing and cancer biology investigations.
• Hybridoma Production: Essential for producing monoclonal antibodies, crucial for diagnostics and therapeutics.
• Cell Line Characterization: Provides a stable environment for the assessment of cell lines’ biological properties and responses to treatments.

Specific Cell Lines Supported During Culturing:

• Jurkat Cells: Frequently used in T-cell studies and immunological research.
• HeLa Cells: A widely studied cervical cancer cell line known for its rapid proliferation.
• MCF-7 Cells: Commonly used breast cancer cells for hormone response and drug sensitivity assays.
• RAW 264.7 Cells: A murine macrophage cell line ideal for inflammation and immune studies.

By employing RPMI 1640 Solution - Modification 2, researchers can achieve consistent and reliable results, driving advancements in cell biology and therapeutic developments.

Disclaimer: Results may vary depending on specific cell lines, specifications, and experimental conditions. Consult with cell culture specialists and review comprehensive literature to tailor its use to your specific research needs.