Roots™ Tissue Culture Media
Roots™ Tissue Culture Media
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Strong Roots. Strong Plants.
Roots™ is a specialized tissue culture medium formulated to promote robust root initiation and development across a wide range of plant species. Designed for use in the rooting phase of micropropagation, Roots™ ensures clean, uniform root systems that support strong acclimatization and successful transfer to ex vitro conditions.
This proprietary blend of essential nutrients, vitamins, and finely tuned plant growth regulators stimulates reliable root formation. Roots™ is the go-to choice for labs seeking consistent, high-quality rooting results in both research and commercial settings.
Available in powder sachets, Roots™ is manufactured to the highest quality standards to ensure clarity, stability, and sterility from batch to batch.
Key Benefits:
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Encourages fast, healthy root development
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Minimizes hyperhydricity
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Enhances survival rates during acclimatization
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Transparent formulation for consistency and reproducibility
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Scalable for both R&D and commercial production
Recommended Use:
Ideal for Stage III (Rooting) in the plant tissue culture lifecycle. For best results, pair with Shoots™ for multiplication and Maintenance Media to support continued growth post-rooting.
Packaging Summary:
Roots™ tissue culture media comes with 10 individual packets, each designed to make 125 mL of prepared media. Just add water, adjust pH, and autoclave for a fast, reliable rooting solution.
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Product Information
Product Information
How to Prepare 125 mL Roots™ Media
- Gather Materials
- 1 sachet Roots™ media (makes 125 mL)
- 125 mL distilled or RO water
- 0.5 g Supreme Tissue Culture Agar
- pH meter or pH paper
- Magnetic stirrer or sterile stir rod
- Autoclavable flask or bottle (for mixing)
- Culture vessels (jars, tubes, or containers)
- Autoclave or Pressure cooker - Dissolve the Media
- In an autoclavable flask, add ~100 mL of - distilled/RO water.
- Pour the entire 125 mL Roots™ sachet into the water.
- Add 0.5 g agar.
- Stir gently until the powder is fully dissolved.
- Add the remaining water to bring the total volume to 125 mL. - Adjust pH
- Measure the pH.
- Adjust to 5.7–5.8 using small drops of 1 N NaOH (to raise) or 1 N HCl (to lower). Alternately, use hydroponics pH up and pH down. - Prepare for Sterilization
- Loosely cap the flask with the prepared media.
- Place your empty culture vessels (jars, tubes, or containers) in the autoclave as well, with loose-fitting lids to allow steam to enter. - Autoclave
- Autoclave the media flask and the empty vessels together at 121 °C (15 psi) for 15–20 minutes. - Pour Media into Sterile Vessels
- After autoclaving, allow the media to cool to about 50–55 °C—still liquid but not too hot.
- In a sterile laminar flow hood, carefully remove the sterilized vessels.
- Pour the cooled, sterile media into the sterile vessels.
- Let the media fully cool and solidify inside the vessels.
- Close the vessels with their sterilized lids. - Inoculate Explants
- Once the media is completely cooled and solidified, place your explants (Stage III rooting) into the vessels under sterile conditions.
- Seal the containers and move them to the proper culture environment.
Key Notes:
- Vessels and media are sterilized in the same autoclave run, saving time.
- Always pour inside a sterile laminar flow hood to avoid contamination.
- Never put explants into warm media.
- Label vessels with date, medium type, and plant name.
Shipping & Storage
Shipping & Storage
| Storage Temp | Room temperature, store away from direct light. |
| Shelf Life | Check the label on the bottle for the expiry date of the product |
| HS Code | 3821.00 |
