December 13, 2025

Online LIVE Tissue Culture Master Class

Tissue Culture Master Class

$999

Hosted by Plant Cell Technology

Contact Host

Date and Time

Sat Dec 13 2025, 1:00 PM - Sun Dec 14 2025, 7:00 PM EST

Location PCT Tissue Culture Lab

Virtual

Francisco Palacios

Meet Francisco Palacios: Tissue Culture Expert, Lab Director, and Master Class instructor at Plant Cell Technology. Fascinated by plant tissue culture and carnivorous plants since age 16, Francisco boasts fourteen years of dedicated experience. Armed with a degree in Plant Molecular Genetics/Biotechnology from the University of Tennessee, he brings innovation and expertise to his role. Francisco's passion extends beyond the lab; he educates globally through platforms like YouTube and TikTok. Join his Master Classes for an immersive journey into plant tissue culture, where learning evolves into community and growth. Explore with Francisco and flourish alongside us.

Event description

The specialty plant industry is evolving faster than ever, and plant tissue culture is the cutting-edge solution to meet surging global demand, ensure genetic stability, and guarantee clean stock. Don’t get left behind, master this essential technology from the comfort of your own home or lab!

After many successful in-person and online master classes, Plant Cell Technology is proud to present our very first Online LIVE Tissue Culture Master Class: Specialty Plant.

This is a comprehensive, two-day virtual deep-dive into the theory and practical application of tissue culture specifically tailored for the specialty plant sectors.

The event will be led by Plant Cell Technology's Tissue Culture Expert and Lab Director, Francisco Palacios, who brings over 14 years of experience in plant tissue culture, crop genetics, and biotechnology.

This class is ideal for growers, lab technicians, dispensary owners, entrepreneurs, and enthusiasts who want to learn how to:

Achieve explosive, contamination-free micropropagation.
Secure long-term genetic preservation of elite strains.
Diagnose and remediate pathogens, including the devastating Hop Latent Viroid (HLVd).

What's Included in the Online Master Class?

This complete virtual package will lift you from the basics of tissue culture and put you at the forefront of specialty plant biotechnology. You’ll get access to cutting-edge protocols and expert knowledge without the high cost of an initial lab setup.

Key features include:

12 Hours of LIVE Instruction (6 hours each day) led by expert Francisco Palacios.
Comprehensive Protocols and material lists for your own future lab work.
Certificate of Completion to showcase your expertise in this advanced field.

Live Q&A Sessions throughout both days to get your specific questions answered.

Detailed Itinerary

Day 1: Foundations and Aseptic Techniques (1:00 PM – 7:00 PM ET)

Time Slot	Topic	Description
1:00 PM – 2:00 PM	Introduction & Principles	What is plant tissue culture? Overview of the critical advantages of specialty plant Tissue Culture (pathogen-free production, genetic stability, speed, and scale).
2:00 PM – 3:30 PM	Tissue Culture Media: The Secret Sauce	Learn the exact requirements for macro/micronutrients, vitamins, and hormones. Hands-on demonstration on how to make tissue culture media and adjusting pH.
3:30 PM – 4:30 PM	Plant Sterilization	Master essential surface sterilization and aseptic techniques. Learn how to handle clean explants and techniques for working with contaminated plants.
4:30 PM – 5:30 PM	Embryo Rescue & Micropropagation	Introduction to advanced techniques like embryo rescue and its application in specialty plant breeding.
5:30 PM – 7:00 PM	Setting Up Your Tissue Culture Lab	Essential equipment, tools, and best practices for building a cost-effective home or commercial lab (laminar flow hoods, pressure cookers, etc.).

Day 2: Cutting-Edge Biotechnology & Pathogen Remediation (1:00 PM – 7:00 PM ET)

Time Slot	Topic	Description
1:00 PM – 2:30 PM	Pathogen Detection & Remediation	In-depth look at major threats like Hop Latent Viroid (HLVd). Learn the basics of running qPCR for pathogen testing and interpreting results.
2:30 PM – 3:30 PM	Remediation & Meristem Dissection	Learn how to use tissue culture to effectively eliminate viruses and viroids. Detailed, step-by-step procedure for shoot apical meristem dissection for pathogen-free clones.
3:30 PM – 4:30 PM	special plant Gender Screening	Introduction to high-throughput, molecular technology to identify male and female plants at a young age, saving weeks of cultivation time.
4:30 PM – 5:30 PM	Long-Term Storage & Conservation	Plant Conservation using tissue culture. Learn how to use synthetic seeds to create stable, long-term storage solutions for valuable genetics.
5:30 PM – 6:00 PM	Deflasking and Acclimatization	The critical final stage: successful deflasking and acclimatization of specialty plant plantlets from in vitro to greenhouse conditions.
6:00 PM – 7:00 PM	Final Q&A Session	Your last chance to ask Francisco and the PCT team your advanced questions.

Hear From Past Attendees:

"I wish that I had taken the class a year ago when I was first getting started. It would have answered so many questions that I had back then. The meristem isolation was the best part—actually being able to refine the skills under the microscope."

"We got more than what we expected, and it has been a thrilling experience for us. I feel more confident now about tissue culture and using the technique to grow my business.”

Seats are limited to ensure a great and personable class experience. Reserve your spot now to secure your access to this essential knowledge!

Terms and Conditions

Plant Cell Technology, Inc reserves the right to cancel or change any class due to insufficient enrollment or other extenuating circumstances. Students enrolled in canceled classes will receive a credit towards the next master class of the same or lesser value. In the event of dates or instructor changes, all students will be notified.

By attending the master class, you agree to the following statement: "Participation in the master class implies acceptance of the understanding that PCT assumes no responsibility for any personal injury or damage to property."

Refund policy: No refunds.