Linsmaier & Skoog (LS) Basal Salts Modification 4

Linsmaier & Skoog (LS) Basal Salts - Modification 4 containing macronutrients, micronutrients, vitamins, and 30 g/L sucrose, buffered to a pH of 5.7 ± 0.2, are crucial for successful plant cell culture. This nutrient-rich medium provides the ideal environment for plant cells to thrive, promoting cell division, differentiation, and regeneration. The addition of sucrose serves as a vital carbon source, supporting energy metabolism and enhancing growth. This formulation is particularly effective for applications such as micropropagation, tissue regeneration, and the study of plant responses to environmental stressors.

Applications of LS Basal Salts - Modification 4 in Plant Cell Culture:

• Micropropagation: Facilitates rapid cloning of desirable plant varieties under sterile conditions.
• Callus Induction and Regeneration: Supports the formation of callus tissue, enabling further development into whole plants.
• Genetic Transformation Studies: Provides a robust platform for integrating foreign DNA into plant cells.
• Stress Physiology Research: Enables the investigation of plant responses to biotic and abiotic stress.

Specific Plant Species Supported by LS Basal Salts - Modification 4:

• Thale Cress ( Arabidopsis thalian)
• Tobacco (Nicotiana tabacum)
• Potato (Solanum tuberosum)
• Banana (Musa spp.)
• Cabbage (Brassica oleracea)

By utilizing LS Basal Salts - Modification 4 researchers and horticulturists can significantly enhance their plant culture techniques, driving advancements in agriculture, conservation, and plant biotechnology.

Disclaimer: Results may vary depending on specific cell lines, specifications, and experimental conditions. Consult with cell culture specialists and review comprehensive literature to tailor its use to your research needs.