Ham's F-12 Medium - Modification 5

Ham's F-12 Medium - Modification 5 containing L-glutamine but not containing phenol red is a specialized cell culture medium that offers a suitable environment for culturing cells without the interference of phenol red dye, which can affect certain assays and experiments. This medium provides a balanced formulation of nutrients, vitamins, and salts necessary for cell growth and maintenance, making it an excellent choice for a variety of cell culture applications where the presence of phenol red is undesirable.

Some specific applications of Ham's F-12 Medium - Modification 5 include:

1. Assay development: used in the development of various cell-based assays where the presence of phenol red could interfere with the assay readouts.
2. Cell viability assays: suitable for cell viability assays that require accurate measurement of cell proliferation and metabolic activity without the confounding effects of phenol red.
3. Fluorescence microscopy: preferred for fluorescence microscopy experiments to avoid background fluorescence interference from the dye.
4. Hormone studies: Researchers studying hormone-sensitive cells may choose this medium to avoid any potential interactions between phenol red and the hormones being studied.

Disclaimer: Results may vary depending on specific cell lines, specifications, and experimental conditions. Consult with cell culture specialists and review comprehensive literature to tailor its use to your specific research needs.