Plant Preservative Mixture (PPM™)
Plant Preservative Mixture (PPM™)
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Plant Preservative Mixture (PPM™), is a broad-spectrum biocide/ for plant tissue culture. PPM™ is the ultimate solution to the never-ending struggle against microbial airborne, waterborne, and endogenous contamination.
Coupled with proper observation of aseptic laboratory techniques, PPM™ can increase your chances of a successful and thriving culture experiment.
The Benefits of Plant Preservative Mixture
- At optimum doses, PPM™, which stands for Plant Preservative Mixture™, is an extremely effective Preservative/Biocide, yet does not impair in vitro seed germination, callus proliferation and callus regeneration.
- PPM™ prevents the germination of both bacteria and fungi spores. It is heat stable and therefore can be autoclaved with the media.
- PPM™ can be, and should be used as a standard ingredient in plant tissue culture media, and is also substantially less expensive than commonly used antibiotics.
- While PPM™ was principally designed to inhibit airborne contamination, waterborne contamination, and contamination introduced from human contact, it can also -- in many cases -- be used to reduce endogenous contamination.
- PPM™ does not affect the genetic profile of the plants.
How To Use Plant Preservative Mixture?
- General Dosage levels:
With the exception of endogenous contamination, the recommended dose range is 0.05%-0.2%. (For callus proliferation, organogenesis and embryogenesis, the recommended range is 0.05-0.075%.) To eliminate higher endogenous contamination densities, higher doses of PPM™ are needed.
- Endogenous Contamination:
(a) For explants: gently and routinely shake / stir 1 cm. long explants (or shorter) for 4-12 hours in 4-5% v/v PPM™ solution supplemented as above with 3x MS
strength basal salts without Tween 20.Without rinsing, insert into a medium supplemented with 0.05 - 0.1% PPM™ for herbaceous plants and 0.2% PPM™ for woody plants.
Note: Paragraphs 6(b) through 10 below are intended for ornamental plants only.
(b)For tubers, bulbs and scales: shake / stir the entire tuber/ bulb / scale in bleach. Rinse with water (can be done under non-sterile conditions). Slice the tuber / bulb /scale to thin slices. Shake / stir for 12-24 hours in 4- 5% PPM™ solution supplemented with full strength basal salts without pH ing & Tween 20. Without rinsing, insert into a medium supplemented with 0.1 -0.2% PPM™.
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Shipping & Storage
Shipping & Storage
Storage Temp | 39° F (4° C). |
Shelf Life | Check the product label for expiration date |
HTS Code | 3808999000 |
Product Information
Product Information
Product Name | Plant Preservative Mixture (PPM™) |
Synonyms | PPM™ |
Feature | Autoclavable and heat stable |
Use | Preservative for plant tissue culture media |
pH | 5 - 7 |
Capacity | Available in different volumes of 30mL, 100mL, 250mL, 500mL, and 1L. |
Shape | Cylindrical container |
Material | Liquid |
Color | Clear to light amber |
Odor | Mild |
Solubility | Infinite in water |
Suitability | For Tissue Culture |
Product Resources
Product Resources
Document:
Video Tutorials
Video Demonstration
Applications
Applications
- Controller of microbial airborne contamination.
- Manager of microbial waterborne contamination.
- Inhibitor of endogenous contamination.
- Enhancer of the success of culture experiments.
- Maintainer of the viability of in vitro seed germination.
- Promoter of callus proliferation.
- Supporter of callus regeneration.
- Ensurer of the stability of tissue culture media.
![Image of PPM™ product packaging: opaque white bottle with PCT logo, 'Plant Preservative Mixture' title, and product details](http://plantcelltechnology.com/cdn/shop/products/PPM__48937.1694176889.1280.1280.png?v=1708200857&width=1445)
![Image of PPM™ bottles of various sizes: opaque white bottle, 'PPM Preservative for Plant Tissue Culture Media' title, and product details.](http://plantcelltechnology.com/cdn/shop/products/2__18357.1683164168.1280.1280.png?v=1708200857&width=1445)
![Image of PPM™ bottles of various sizes: opaque white bottle, 'PPM Preservative for Plant Tissue Culture Media' title, and product details.](http://plantcelltechnology.com/cdn/shop/products/3__52715.1683164176.1280.1280.png?v=1708200857&width=1445)
![Image of PPM™ large size: opaque white bottle, with PCT Logo, 'PPM Preservative for Plant Tissue Culture Media' title, and product details.](http://plantcelltechnology.com/cdn/shop/products/8__38246.1683164184.1280.1280.png?v=1708200857&width=1445)
![Image of PPM™ large size: opaque white bottle, with PCT Logo, 'PPM Preservative for Plant Tissue Culture Media' title, and product details.](http://plantcelltechnology.com/cdn/shop/products/7__80561.1683164187.1280.1280.png?v=1708200857&width=1445)
![Image of PPM™ large size: opaque white bottle, with PCT Logo, 'PPM Preservative for Plant Tissue Culture Media' title, and product details.](http://plantcelltechnology.com/cdn/shop/products/6__63520.1683164190.1280.1280.png?v=1708200857&width=1445)
![Image of PPM™ medium size: opaque white bottle, with PCT Logo, 'PPM Preservative for Plant Tissue Culture Media' title, and product details.](http://plantcelltechnology.com/cdn/shop/products/5__90892.1683164193.1280.1280.png?v=1708200857&width=1445)
![Image of PPM™ small size: opaque white bottle, with PCT Logo, 'PPM Preservative for Plant Tissue Culture Media' title, and product details.](http://plantcelltechnology.com/cdn/shop/products/4__60260.1683164195.1280.1280.png?v=1708200857&width=1445)
![Image of PPM™ bottles in descending size order: opaque white bottles featuring the PCT logo, 'PPM Preservative for Plant Tissue Culture Media' title, and product details.](http://plantcelltechnology.com/cdn/shop/products/IMG_2734_jpg__34140.1683164230.1280.1280.jpg?v=1708200857&width=1445)