Starter Kit

Square Tissue Culture Vessels with Lids:

• Description: Heat Resistant and autoclavable.
• Feature: Lids Included
• Capacity: Plant growth field of 6.15 square inches
• Shape: squared container
• Color: transparent
• Measurements: 3" x 3" x 4"

Supreme Tissue-Culture Grade Agar

• Description: A polysaccharide complex obtained through bleaching and hot water extraction of agarocytes from the red alga Rhodophyceae. Typical usage rate of 6 - 12 g/L medium. Supreme agar offers greater clarity of plant culture media.
• Gel Strength: 1050 GM/CM^2
• Packaging: 100g, 500g, 1Kg in poly bottles
• Application: Agar is used as microbial solid culturing media. The substance is often used in doses ranging between 0.5 to 1.0% (w/v) to solidify plant tissue culture media. Agar is an essential ingredient used for in vitro root development.

Murshige & Skoog Medium

• Description: MURASHIGE & SKOOG MEDIUM  (W/ VITAMINS & CALCIUM CHLORIDE, W/O SUCROSE, AGAR)
• Product Technical Details: Support s or facilitate plant growth and/or shoot proliferation in two or more plant tissue cultures (both in monocyledons and dicotyledons).
• FORMULA WT.: 4.54 GMS/LTR
• Instructions for Use: Dissolve 4.54gms of dehydrated medium in 600ml of distilled or deionized water at room temperature (15-30*C). Rinse media vial with small quantity of distilled water to remove traces of power. Add the desired heat stable supplements prior to autoclaving. Continue stirring until the powder has dissolved. Some times media does not dissolve completely unless the pH is reduced. For these, lower the pH to about 3.0 to facilitate dissolution of media. The pH of medium is adjusted by using 1N HCL/ 1N NaOH/ 1NKOH. Make up the final volume to 1000ml with distilled water. Mix Gently, heat and rotate between intervals until the solution becomes clear. Do not boil, reheat and allow to cool below 50*C during dispensing. Dispense the medium into suitable containers, plug or cap, then autoclave at 15pso (121*C) for 15 minutes, using a slow exhaust cycle. Higher temperatures and/or longer times are not recommended. Cool the autoclaved culture vessels containing medium to 45-50*C and aseptically add desired sterile heat-labile substrate. Note: Media should be prepared according to formula mentioned on the label, however, it is recommended to use an entire container at once. Heat-liable substrates should be added, after autoclaving.
• Storage and Shelf Life: Dehydrated plant tissue culture media is hygroscopic and should be protected from sunlight and moisture. Store the prepared medium at 2-8*C away from direct light. Medium should be used before expiry date. The entire volume of each bottle should be used immediately after opening or else the unused portion should be stored in desiccators and refrigerated at 2-8*C.
• Product currently in back order. All purchases will be pre-order until product is received.

Plant Preservative Mixture (PPM™):

• Description: PPM™ is a heat stable preservative/biocide which can be used to effectively prevent or reduces microbial contamination in plant tissue culture.
• At optimum doses, PPM™, which stands for Plant Preservative Mixture™, is an extremely effective Preservative/Biocide, yet does not impair in vitro seed germination, callus proliferation and callus regeneration.
• PPM™ prevents the germination of both bacteria and fungi spores. It is heat stable and therefore can be autoclaved with the media
• PPM™ can be, and should be used as a standard ingredient in plant tissue culture media, and is also substantially less expensive than commonly used antibiotics.
• Despite the most stringent use of sterile techniques, the contamination of plant cell and plant tissue cultures remain a persistent problem that can result in losses ranging from small number of cultures to the loss of whole batches.
• While PPM™ was principally designed to inhibit airborne contamination, waterborne contamination and contamination introduced from human contact, it can also -- in many cases -- be used to reduce endogenous contamination.

Physical Properties:

The PCT Starter Kit facilitates propagation of rare species, genetic modifications, and advanced plant biology techniques for enthusiasts and researchers alike.

Documents:

• MS Media MSDS
• Supreme TC Grade Agar MSDS
• PPM™ MSDS
• Inquire for Certificate Of Analysis