null

Starter Kit

TSh203,388.53
(4 reviews) Write a Review
SKU:
STRBDL
Weight:
1.80 LBS
Width:
7.00 (in)
Height:
4.00 (in)
Depth:
5.00 (in)
Current Stock:

 

WHAT IS INSIDE THE PCT STARTER KIT ?

Are you interested in Tissue Culture but are unsure where to start? The PCT Starter Kit is PERFECT chance for you to try out and hone your new skillset!

In this bundle you will receive:

 

  • MS Media with Vitamins: Make 5L of tissue culture media using the kit.
  • 100 grams Supreme Tissue-Culture-Grade Agar: Provide the support your plants need.
  • 10 ml PPM™: Fight against all kinds of contamination and protect your cultures.
  • 2x Squared Culture Vessels: Provide the perfect environment for your tissue culture plants to grow and develop.
  • 1 Disposable Syringe: Easily Measure Your Ingredients for the Perfect Tissue Culture Recipe.

 

ABOUT SQUARED TISSUE CULTURE VESSELS WITH LIDS:

Description: Heat Resistant and autoclavable.

Feature: Lids Included

Capacity: Plant growth field of 6.15 square inches

Shape: squared container

Color: transparent

Measurements: 3" x 3" x 4"

 

ABOUT SUPREME TISSUE-CULTURE-GRADE AGAR:

Description:
A polysaccharide complex obtained through bleaching and hot water extraction of agarocytes from the red alga Rhodophyceae. Typical usage rate of 6 - 12 g/L medium. Supreme agar offers greater clarity of plant culture media.
 
Gel Strength:

1050 GM/CM^2

Packaging:

100g, 500g, 1Kg in poly bottles

Application:

Agar is used as microbial solid culturing media. The substance is often used in doses ranging between 0.5 to 1.0% (w/v) to solidify plant tissue culture media. Agar is an essential ingredient used for in vitro root development.

Documents:
 

ABOUT MS MEDIA WITH VITAMINS:

MURASHIGE & SKOOG MEDIUM  (W/ VITAMINS & CALCIUM CHLORIDE, W/O SUCROSE, AGAR)

 Product Technical Details:

Supports or facilitate plant growth and/or shoot proliferation in two or more plant tissue cultures (both in monocyledons and dicotyledons).

INGREDIENTS

QUANITY (MG/LTR)

   

POTASSIUM PHOSPHATE MONOBASIC

170

POTASSIUM NITRATE

1900

MAGNESIUM SULPHATE

180.69

AMMONIUM NITRATE

1650

COBALT CHLORIDE 6H2O

0.025

COPPER SULPHATE 5H2O

0.025

BORIC ACID

6.2

EDTA DISODIUM SALT 2H2O

37.3

POTASSIUM IODIDE

0.83

MANGANESE SULFATE H2O

16.9

FERROUS SULPHATE 7H2O

27.8

SODIUM MOLYBDATE 2H2O

0.25

ZINC SULPHATE 7H2O

8.6

CALCIUM CHLORIDE 2H2O

440

GLYCINE

2

MYO-INOSITOL

100

PYRIDOXINE HCI

0.5

THIAMINE HCI

0.1

NICOTINIC ACID

0.5


**FORMULA WT. 4.54 GMS/LTR**

 Physical Properties:

Appearance

White to light tan with homogenous mixture of free flowing powder

Solubility

Colorless to slight yellow solution, clear, complete

pH (at 25*C)

4.8      0.2

Product currently in back order. All purchases will be pre-order until product is received.  

Instructions for Use:

 Dissolve 4.54gms of dehydrated medium in 600ml of distilled or deionized water at room temperature (15-30*C). Rinse media vial with small quantity of distilled water to remove traces of power. Add the desired heat stable supplements prior to autoclaving. Continue stirring until the powder has dissolved. Some times media does not dissolve completely unless the pH is reduced. For these, lower the pH to about 3.0 to facilitate dissolution of media. The pH of medium is adjusted by using 1N HCL/ 1N NaOH/ 1NKOH. Make up the final volume to 1000ml with distilled water. Mix Gently, heat and rotate between intervals until the solution becomes clear. Do not boil, reheat and allow to cool below 50*C during dispensing. Dispense the medium into suitable containers, plug or cap, then autoclave at 15pso (121*C) for 15 minutes, using a slow exhaust cycle. Higher temperatures and/or longer times are not recommended. Cool the autoclaved culture vessels containing medium to 45-50*C and aseptically add desired sterile heat-labile substrate.

 Note: Media should be prepared according to formula mentioned on the label, however, it is recommended to use an entire container at once. Heat-liable substrates should be added, after autoclaving.

 Storage and Shelf Life:

 Dehydrated plant tissue culture media is hygroscopic and should be protected from sunlight and moisture. Store the prepared medium at 2-8*C away from direct light. Medium should be used before expiry date. The entire volume of each bottle should be used immediately after opening or else the unused portion should be stored in desiccators and refrigerated at 2-8*C.

ABOUT PLANT PRESERVATIVE MIXTURE (PPM™):

PPM™ is a heat stable preservative/biocide which can be used to effectively prevent or reduces microbial contamination in plant tissue culture

  • At optimum doses, PPM™, which stands for Plant Preservative Mixture™, is an extremely effective Preservative/Biocide, yet does not impair in vitro seed germination, callus proliferation and callus regeneration.
  • PPM™ prevents the germination of both bacteria and fungi spores. It is heat stable and therefore can be autoclaved with the media
  • PPM™ can be, and should be used as a standard ingredient in plant tissue culture media, and is also substantially less expensive than commonly used antibiotics

Despite the most stringent use of sterile techniques, the contamination of plant cell and plant tissue cultures remain a persistent problem that can result in losses ranging from small number of cultures to the loss of whole batches.

While PPM™ was principally designed to inhibit airborne contamination, waterborne contamination and contamination introduced from human contact, it can also -- in many cases -- be used to reduce endogenous contamination.

 

  • 5
    Awesome kit unbelievable price

    Posted by Michael Lazar on Jul 14, 2022

    This is a great way to get into tissue culture and to learn it on a budget. You can not go wrong this is going to be the future of gardening. Tissue culture clones and seeds and a great way to save and preserve your old rare seed stock. Don't pass up on this kit at this price you can not go wrong!!!

  • 5
    Easiest starter kit

    Posted by Ellie Bagwell on Jul 14, 2022

    I was always nervous to start tissue culture but this PCT starter bundle made it so easy to understand. I'm so happy to start my tissue culture journey. The PCT starter bundle is definitely the way to get into tissue culture.

  • 5
    Excited to get started

    Posted by Steve on Jun 08, 2021

    Received everything as described. Make sure to read the card that comes with the kit since that's where the QR code to the instructions is. I'm looking forward to trying it out!

  • 5
    PCT Starter kit

    Posted by Jay Horn on Mar 11, 2021

    very pleased with this product! haven't used it yet , but currently doing TC at work.This kit is for home use.which was very.neatly packed. also the culture vessel cover with the vents at the top are a bonus! thank you and great work to the PCT team!