
Plant Preservative Mixture (PPM)
Feature: Heat resistant
Capacity: 250ml
Shape: cylindrical container
Material: Borosilicate Glass 3.3
Color: transparent
Width: 2.60 In
Height: 3.50 In
Diameter: 2.60 In
1050 GM/CM^2
100g, 500g, 1Kg in poly bottles
Agar is used as microbial solid culturing media. The substance is often used in doses ranging between 0.5 to 1.0% (w/v) to solidify plant tissue culture media. Agar is an essential ingredient used for in vitro root development.
MURASHIGE & SKOOG MEDIUM (W/ VITAMINS & CALCIUM CHLORIDE, W/O SUCROSE, AGAR)
Product Technical Details:
Supports or facilitate plant growth and/or shoot proliferation in two or more plant tissue cultures (both in monocyledons and dicotyledons).
INGREDIENTS |
QUANITY (MG/LTR) |
POTASSIUM PHOSPHATE MONOBASIC |
170 |
POTASSIUM NITRATE |
1900 |
MAGNESIUM SULPHATE |
180.69 |
AMMONIUM NITRATE |
1650 |
COBALT CHLORIDE 6H2O |
0.025 |
COPPER SULPHATE 5H2O |
0.025 |
BORIC ACID |
6.2 |
EDTA DISODIUM SALT 2H2O |
37.3 |
POTASSIUM IODIDE |
0.83 |
MANGANESE SULFATE H2O |
16.9 |
FERROUS SULPHATE 7H2O |
27.8 |
SODIUM MOLYBDATE 2H2O |
0.25 |
ZINC SULPHATE 7H2O |
8.6 |
CALCIUM CHLORIDE 2H2O |
440 |
GLYCINE |
2 |
MYO-INOSITOL |
100 |
PYRIDOXINE HCI |
0.5 |
THIAMINE HCI |
0.1 |
NICOTINIC ACID |
0.5 |
**FORMULA WT. 4.54 GMS/LTR**
Physical Properties:
Appearance |
White to light tan with homogenous mixture of free flowing powder |
Solubility |
Colorless to slight yellow solution, clear, complete |
pH (at 25*C) |
4.8 0.2 |
Dissolve 4.54gms of dehydrated medium in 600ml of distilled or deionized water at room temperature (15-30*C). Rinse media vial with small quantity of distilled water to remove traces of power. Add the desired heat stable supplements prior to autoclaving. Continue stirring until the powder has dissolved. Some times media does not dissolve completely unless the pH is reduced. For these, lower the pH to about 3.0 to facilitate dissolution of media. The pH of medium is adjusted by using 1N HCL/ 1N NaOH/ 1NKOH. Make up the final volume to 1000ml with distilled water. Mix Gently, heat and rotate between intervals until the solution becomes clear. Do not boil, reheat and allow to cool below 50*C during dispensing. Dispense the medium into suitable containers, plug or cap, then autoclave at 15pso (121*C) for 15 minutes, using a slow exhaust cycle. Higher temperatures and/or longer times are not recommended. Cool the autoclaved culture vessels containing medium to 45-50*C and aseptically add desired sterile heat-labile substrate.
Note: Media should be prepared according to formula mentioned on the label, however, it is recommended to use an entire container at once. Heat-liable substrates should be added, after autoclaving.
Storage and Shelf Life:
Dehydrated plant tissue culture media is hygroscopic and should be protected from sunlight and moisture. Store the prepared medium at 2-8*C away from direct light. Medium should be used before expiry date. The entire volume of each bottle should be used immediately after opening or else the unused portion should be stored in desiccators and refrigerated at 2-8*C.
PPM™ is a heat stable preservative/biocide which can be used to effectively prevent or reduces microbial contamination in plant tissue culture
Despite the most stringent use of sterile techniques, the contamination of plant cell and plant tissue cultures remain a persistent problem that can result in losses ranging from small number of cultures to the loss of whole batches.
While PPM™ was principally designed to inhibit airborne contamination, waterborne contamination and contamination introduced from human contact, it can also -- in many cases -- be used to reduce endogenous contamination.
very pleased with this product! haven't used it yet , but currently doing TC at work.This kit is for home use.which was very.neatly packed. also the culture vessel cover with the vents at the top are a bonus! thank you and great work to the PCT team!