Starter Kit

WHAT IS INSIDE THE PCT STARTER KIT ?

Are you interested in Tissue Culture but are unsure where to start? The PCT Starter Kit is PERFECT chance for you to try out and hone your new skillset!

In this bundle you will receive:

- 3 x 250ml Glass Tissue Culture Vessels

- 100g of Agar Powder

- 5L of M.S. Media Powder

- 10ml of PPM

ABOUT 250ML GLASS TISSUE CULTURE VESSELS:

Feature: Heat resistant

Capacity: 250ml

Shape: cylindrical container

Material: Borosilicate Glass 3.3

Color: transparent

ABOUT AGAR POWDER:

ABOUT MS MEDIA:

MURASHIGE & SKOOG MEDIUM  (W/ VITAMINS & CALCIUM CHLORIDE, W/O SUCROSE, AGAR)

 Product Technical Details:

Supports or facilitate plant growth and/or shoot proliferation in two or more plant tissue cultures (both in monocyledons and dicotyledons).

**FORMULA WT. 4.54 GMS/LTR**

 Physical Properties:

Instructions for Use:

 Dissolve 4.54gms of dehydrated medium in 600ml of distilled or deionized water at room temperature (15-30*C). Rinse media vial with small quantity of distilled water to remove traces of power. Add the desired heat stable supplements prior to autoclaving. Continue stirring until the powder has dissolved. Some times media does not dissolve completely unless the pH is reduced. For these, lower the pH to about 3.0 to facilitate dissolution of media. The pH of medium is adjusted by using 1N HCL/ 1N NaOH/ 1NKOH. Make up the final volume to 1000ml with distilled water. Mix Gently, heat and rotate between intervals until the solution becomes clear. Do not boil, reheat and allow to cool below 50*C during dispensing. Dispense the medium into suitable containers, plug or cap, then autoclave at 15pso (121*C) for 15 minutes, using a slow exhaust cycle. Higher temperatures and/or longer times are not recommended. Cool the autoclaved culture vessels containing medium to 45-50*C and aseptically add desired sterile heat-labile substrate.

 Note: Media should be prepared according to formula mentioned on the label, however, it is recommended to use an entire container at once. Heat-liable substrates should be added, after autoclaving.

 Storage and Shelf Life:

 Dehydrated plant tissue culture media is hygroscopic and should be protected from sunlight and moisture. Store the prepared medium at 2-8*C away from direct light. Medium should be used before expiry date. The entire volume of each bottle should be used immediately after opening or else the unused portion should be stored in desiccators and refrigerated at 2-8*C.

ABOUT PLANT PRESERVATIVE MIXTURE (PPM):

PPM™ is a heat stable preservative/biocide which can be used to effectively prevent or reduces microbial contamination in plant tissue culture

• At optimum doses, PPM™, which stands for Plant Preservative Mixture™, is an extremely effective Preservative/Biocide, yet does not impair in vitro seed germination, callus proliferation and callus regeneration.
• PPM™ prevents the germination of both bacteria and fungi spores. It is heat stable and therefore can be autoclaved with the media
• PPM™ can be, and should be used as a standard ingredient in plant tissue culture media, and is also substantially less expensive than commonly used antibiotics

Despite the most stringent use of sterile techniques, the contamination of plant cell and plant tissue cultures remain a persistent problem that can result in losses ranging from small number of cultures to the loss of whole batches.

While PPM™ was principally designed to inhibit airborne contamination, waterborne contamination and contamination introduced from human contact, it can also -- in many cases -- be used to reduce endogenous contamination.