Murashige & Skoog (MS) Basal Medium w/ Vitamins
Murashige & Skoog (MS) Basal Medium w/ Vitamins
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What is MS Media?
MS or Murashige and Skoog Media is a nutrient media used for the in vitro culture of plant cells, tissues, and organs. It was first developed in 1962 by Toshio Murashige and Folke Skoog and has since become one of the most widely used media for plant tissue culture.
It contains a balanced mixture of macronutrients, micronutrients, vitamins, and plant growth regulators to provide optimal conditions for the proper growth and development of plants.
The Benefits of MS Media
- Easy to use
- Supply nutrients required by in vitro plants for their proper growth and development
- Provides a well-balanced composition of nutrients, including essential minerals and vitamins, supporting the growth of various plant tissues.
- Effective in inducing callus formation
- A dynamic supplier of numerous amino acids, organic acids, plant growth regulators (such as cytokinins and auxins), vitamins, sugars, minerals, nucleic acids, and unidentified growth-promoting substances.
How To Use MS Media?
Follow the given steps to prepare the tissue culture media using MS media:
- Take a beaker and add 800 ml of sterile distilled water to it.
- To the water add PCT’s MS Media 4.54 grams (you don’t need to measure it as it comes in a measured bottle), 30 grams of sugar, and PPM™ (generally 1-2 ml/L of media based on the plants and contamination level.
- Add water to make 950 ml of the volume of the media.
- Adjust the pH of the media between 5.8-6.0.
- Add 8 grams of PCT’s Supreme Tissue Culture Grade Agar to the media and keep stirring.
- Make up the volume of the media to 1000ml.
- Autoclave the medium for 20 minutes at 15 psi and 121℃.
Your media is ready!
Note: Media should be prepared according to the formula mentioned on the label. However, it is recommended to use an entire container at once.
Heat-liable substrates should be added, after autoclaving.
Product Name | Murashige & Skoog (MS) Basal Medium w/Vitamins. |
CAS No. | 1214-39-7 |
Shelf Life | See expiry date on the bottle |
Capacity | Available in volumes of 5L, 10L, 50L, and 100L |
Shape | Cylindrical container |
Material | Liquid |
Solubility | Partially soluble in cold water and completely soluble in hot water |
Color | White to light tan homogenous mixture of free-flowing powder |
Storage Temp | at 2-8 ℃ and away from direct light |
HTS Code | 38210000 |
Documents
Video Tutorial
What is MS Media?
MS or Murashige and Skoog Media is a nutrient media used for the in vitro culture of plant cells, tissues, and organs. It was first developed in 1962 by Toshio Murashige and Folke Skoog and has since become one of the most widely used media for plant tissue culture.
It contains a balanced mixture of macronutrients, micronutrients, vitamins, and plant growth regulators to provide optimal conditions for the proper growth and development of plants.
The Benefits of MS Media
- Easy to use
- Supply nutrients required by in vitro plants for their proper growth and development
- Provides a well-balanced composition of nutrients, including essential minerals and vitamins, supporting the growth of various plant tissues.
- Effective in inducing callus formation
- A dynamic supplier of numerous amino acids, organic acids, plant growth regulators (such as cytokinins and auxins), vitamins, sugars, minerals, nucleic acids, and unidentified growth-promoting substances.
How To Use MS Media?
Follow the given steps to prepare the tissue culture media using MS media:
- Take a beaker and add 800 ml of sterile distilled water to it.
- To the water add PCT’s MS Media 4.54 grams (you don’t need to measure it as it comes in a measured bottle), 30 grams of sugar, and PPM™ (generally 1-2 ml/L of media based on the plants and contamination level.
- Add water to make 950 ml of the volume of the media.
- Adjust the pH of the media between 5.8-6.0.
- Add 8 grams of PCT’s Supreme Tissue Culture Grade Agar to the media and keep stirring.
- Make up the volume of the media to 1000ml.
- Autoclave the medium for 20 minutes at 15 psi and 121℃.
Your media is ready!
Note: Media should be prepared according to the formula mentioned on the label. However, it is recommended to use an entire container at once.
Heat-liable substrates should be added, after autoclaving.
Product Name | Murashige & Skoog (MS) Basal Medium w/Vitamins. |
CAS No. | 1214-39-7 |
Shelf Life | See expiry date on the bottle |
Capacity | Available in volumes of 5L, 10L, 50L, and 100L |
Shape | Cylindrical container |
Material | Liquid |
Solubility | Partially soluble in cold water and completely soluble in hot water |
Color | White to light tan homogenous mixture of free-flowing powder |
Storage Temp | at 2-8 ℃ and away from direct light |
HTS Code | 38210000 |
Documents
Video Tutorial
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I recommend you get all you tc products from them ! And fast delivery which is always a huge plus
Thanks for shipping this media promptly to New Zealand. Ive found this really easy to use and effective in my tissue culture media. Lots of info always available on your website .
I highly recommend Plant Cell Technology for your MS and PPM products for multiple reasons... Firstly the products are extremely potent and high quality and fresh because my plans are responding extremely well! Secondly customer service is extremely helpful and will help you with anything you need help with with nutrients or liquid mediums or whatever it is there's always someone there to help us with every question and any situation! Third of all the delivery is super fast and it gets packaged very well with mixing instructions and storing instructions and everything you can possibly think of, plant cell technology makes sure that their clients receive the best possible quality product and the best experience when shopping on their website! I absolutely love them and I highly recommend them to anyone including my friends